目录
摘要III
关键词III
AbstractIV
引言
引言1
1 荧光探针识别原理1
1.1 荧光探针及荧光检测技术1
1.2 荧光分子探针的设计原则2
1.2.1 共轭结构的影响2
1.2.2 取代基的影响2
1.2.3 推拉电子体系的影响2
1.3 荧光分子探针的设计机理2
1.3.1 分子内电荷转移2
1.3.2 扭曲的分子内电荷转移3
1.3.3 荧光共振能量转移4
1.3.4 光诱导电子转移4
1.4 粘度荧光探针最新应用5
1.4.1 基于香豆素的粘度荧光探针5
1.4.2 基于咔唑基团的粘度荧光探针6
1.4.3 基于萘酰亚胺染料的粘度荧光探针6
1.4.4 基于半花菁染料的粘度荧光探针6
1.5 本研究工作指导思想6
2 探针的合成与检测7
2.1 仪器与原料7
2.2 仪器的设计与合成7
2.3 探针ASI1,ASI2的相关检测8
2.3.1 探针ASI1,ASI2的1HNMR表征8
2.3.2 探针ASI1,ASI2的光谱学特性研究8
2.3.3 探针ASI1,ASI2的荧光强度与粘度间标准曲线的建立
8
2.4 结果与讨论8
2.4.1 探针ASI1,ASI2的1HNMR表征8
2.4.2 探针ASI1,ASI2的光谱学特性9
2.4.3 探针ASI1,ASI2的荧光强度与粘度间标准曲线
10
2.5 本章小结10
3 讨论与展望12
致谢13
参考文献14
附录16
基于TICT机理的转子型探针用于线粒体粘度检测
摘 要
线粒体粘度变化会对细胞正常的生命活动有着很大的影响，如果线粒体粘度不处于正常范围则会影响正常的细胞功能甚至导致 *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
疾病。常见的如糖尿病，肌无力，白内障乃至学习障碍等疾病均与其有关。所以，寻找一种能够准确测定线粒体内粘度变化的方法具有重要意义。以往常用的检测技术是放射性核素标记技术，但其会造成标记物的化学损伤与自身辐射损伤，而新兴的荧光探针技术解决了这个问题。荧光探针技术具有灵敏度高与可视性强等优点，是检测细胞内参数变化以及相关系数的重要工具。荧光探针分子通常具有很大的可塑性，即我们可以根据具体需要来构建符合要求的相关分子，然后通过构建的荧光探针分子进行相关检测。本文基于荧光分子探针的TICT机理，设计了用对二甲氨基苯甲醛为主要反应原料，通过缩合反应构筑的“转子”型探针，该探针对溶液粘度有“开关”型响应并具有良好的抗干扰性和灵敏度，有望进一步用于实际生物细胞内线粒体粘度变化的实时监测。
ROTOR PROBES BASED ON TICT MECHANISM FOR DETECTING MITOCHONDRIAL VISCOSITY
ABSTRACT
The change of mitochondrial viscosity will have a great impact on the normal activities of cells. If the mitochondrial viscosity is not within a normal range, it will affect cell functions and even lead to diseases. Common diseases such as diabetes, muscle weakness, cataracts and even learning disabilities are all associated with it. Therefore, it is of great research significance to find a method that can accurately measure the relative changes of the viscosity in mitochondria. In the past, the commonly used detection technology is the radionuclide labeling technology, which studies the reaction process with the help of isotopic atoms, but it will cause chemical damage to the markers and its own radiation damage. However, the newly developed fluorescent probe technology has solved this problem. It has advantages of high sensitivity and high visibility and is now an important tool for detecting the changes of intracellular parameters and correlation coefficients. Fluorescent probes usually have great plasticity, that is, we can construct relevant fluorescent probes according to specific needs, and then conduct relevant detection through the constructed fluorescent probe molecules. In this article, fluorescent probes based on TICT mechanism was designed to build the "rotor" type of probes by condensation reaction using dimethyl amino benzene formaldehyde as the main starting material. The probes have great "off on" type response to viscosity changes in solution and good antiinterference and sensitivity. The probes are expected to be further used for realtime monitoring of biological cell mitochondria viscosity changes in real samples.

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