目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言
1 芘丁酸卟啉荧光探针的构造1
方案论证1
1.1.1 芘在荧光探针中的应用2
1.1.2 构建FRET能量体系3
1.2 主要实验仪器 4
1.3 实验步骤4
1.4 用卟啉对芘丁酸进行荧光滴定4
1.5 向芘丁酸卟啉体系的PBS溶液中进行肝素等多糖的滴定5
1.5.1 探针PyBA PP1与肝素等多糖的滴定6
1.5.2 探针PyBA PP2与肝素等多糖的滴定7
1.5.3 探针PyBA PP4与肝素等多糖的滴定8
1.5.4 三种探针与肝素结合时间的测定9
2 荧光阵列传感器的构造以及肝素和其他多糖和生物分子检测9
2.1 主要实验仪器9
2.2 实验步骤9
2.3 构建荧光阵列传感器对肝素进行检测10
2.3.1 荧光阵列传感器对不同浓度的多糖的检测10
2.3.2 荧光阵列传感器对不同浓度的生物分子的检测11
2.3.3 荧光阵列传感器在血清中对肝素等多糖及生物分子的检测12
2.3.4 荧光阵列传感器对不同污染程度的肝素溶液的检测14
2.3.5 荧光阵列传感器鉴定未知GAGs样品15
3 讨论17
3.1 对于滴定体系的分析 17
3.2 对于荧光阵列传感器构建的分析 17
3.3 展望 18
致谢18
参考文献18
本科期间发表的论文19
基于芘卟啉体系荧光阵列传感器的开发与肝素检测应用
摘 要
肝素是一种天然的糖胺聚糖，存在于动物组织中，具有多种生物活性。肝素在临床上作为药物被广泛使用，尤其是作为抗凝血剂用于外科手术和透析仪器的防凝血处理。然而肝素的化学结构与其他糖胺聚糖家族成员如透明质酸和硫酸软骨素等非常相似，并且从动物组织中提取的肝素可能混有其他糖胺聚糖类似物杂质，因此发展有效的 *51今日免费论文网|www.51jrft.com +Q: ^351916072^ 
分析方法用于检测肝素至关重要。荧光传感技术因其高灵敏度、远程监测和实时性等优点在分析领域被广泛应用。这方面，芘是一种简单的芳香化合物，具有荧光寿命较长、荧光量子产率较高等特点。它在溶液中可通过形成动态和静态的激基复合物在长波长处出现荧光发射峰，因而常被用作荧光团来合成各种化学传感器。另一方面，卟啉化合物的性质稳定、且荧光发射波长位于近红外光区，可以作为芘的能量受体，构建FRET传感体系。本论文成功开发了由三个芘丁酸卟啉体系探针组成的荧光阵列传感器，并实现了对肝素等多种糖胺聚糖的检测及识别。
 DEVELOPMENT OF RATIOMETRIC FLUORESCENT SENSOR ARRAY BASED ON PYRENE AND PORPHYRIN FOR HEPARIN SENSING
ABSTRACT
Heparin is a natural glycosaminoglycan that exists in animal tissues and has a variety of biological activities. Heparin is widely used clinically as a drug, especially as an anticoagulant for treatment of surgical and dialysis instruments. However, the chemical structure of heparin is very similar to other members of the glycosaminoglycan family, such as hyaluronic acid and chondroitin sulfate, and heparin extracted from animal tissues may be mixed with other glycosaminoglycan analog impurities. So the development of effective analytical methods for heparin sensing is essential. Fluorescence sensing technology is widely used in the analysis field due to its advantages such as high sensitivity, remote monitoring and realtime performance. In this respect, pyrene is a simple aromatic compound with long fluorescence lifetime and high fluorescence quantum yield. It can form dynamic and static excimer associations in solution to generate fluorescence emission peaks at long wavelengths, so it is often used as a fluorophore to synthesize various chemical sensors. On the other hand, porphyrin compounds are stable in nature and have fluorescence emission wavelengths in the nearinfrared region, which can be used as energy acceptors for pyrene to construct FRET sensing systems. In this thesis, a fluorescent sensor array composed of three probes of pyreneporphyrin system was successfully developed, and the detection and recognition of various glycosaminoglycans such as heparin was achieved.

原文链接：http://www.jxszl.com/hxycl/yyhx/607103.html