目录
摘要Ⅱ
关键词Ⅱ
ABSTRACTⅢ
KEY WORDSⅢ
引言
1 材料与方法1
材料1
方法1
水稻幼苗培养1
RNA提取1
反转录1
实时荧光定量PCR1
GUS材料构建及GUS染色2
2 结果与分析2
2.1 OsPDRx在水稻各组织中的定量表达2
2.2 OsPDRx受MeJA诱导后的表达3
2.3 OsPDRx在水稻组织中的定位3
2.4 OsPDRx基因功能初步研究4
3 讨论5
致谢6
参考文献6
水稻ABC转运蛋白OsPDRx的表达特征及功能初步分析
摘 要
ABC转运蛋白普遍存在于植物中，参与多种底物分子的运输，对植物的多种生理活动直接或间接产生影响。迄今有关水稻中ABC转运蛋白的研究较为有限。本研究旨在初步分析水稻（Oryza sativa）ABC转运蛋白OsPDRx的表达特征和功能。通过提取水稻不同生长时期的不同组织的RNA，反转录并进行实时荧光定量PCR发现水稻茎中OsPDRx基因的相对表达量最高，其次是老根。OsPDRx基因在幼根、幼叶、老叶和幼穗中也有不同程度的表达，并且老根和老叶中OsPDRx基因的相对表达量较幼根和幼叶有显著提高。MeJA（茉莉酸甲酯）处理后的水稻幼苗在6小时内OsPDRx基因的相对表达量有显著提高，显示了OsPDRx基因的表达受MeJA的诱导。通过构建GUS转基因材料并进行GUS染色切片观察后发现OsPDRx基因在水稻叶片及根中均有表达，且主要表达于根的中柱以及叶片的维管束。通过对幼苗的表型鉴定发现,ospdr突变体植株的地上部和根部的长度和鲜重均大于野生型，表明OsPDRx基因负向调控水稻幼苗的生长。本研究为深入探索OsPDRx基因的功能打下了基础。
EXPRESSION AND FUNCTION ANALYSIS OF ATPBINDING CASSETTE TRANSPORTER OSPDRX IN ORYZA SATIVA
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TRACT
ABC transporters are ubiquitous in plants, which participate in the transport of many kinds of substrate molecules, and directly or indirectly affect many physiological activities of plants. So far, the research on ABC transporters in Oryza sativa is limited. The purpose of this study was to analyze the expression and function of Oryza sativa ABC transporter OsPDRx. The results showed that the relative expression of OsPDRx was the highest in stem, followed by that in old root. OsPDRx was also expressed in young roots, young leaves, old leaves and young spikes, and the relative expression of OsPDRx in old roots and old leaves was significantly higher than that in young roots and young leaves. The relative expression of OsPDRx in seedlings treated with MeJA was significantly increased within 6 hours, indicating that the expression of OsPDRx was induced by MeJA. It was found that OsPDRx was expressed in leaves and roots, mainly in the middle column of roots and vascular bundles of leaves. Through the phenotypic identification of the seedlings, it was found that the length and fresh weight of the shoots and roots of the ospdr mutant were larger than those of the wild type, indicating that the OsPDRx negatively regulated the growth of rice seedlings. This study laid a foundation for further exploring of the function of OsPDRx.

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