目录
摘要I
关键词I
AbstractII
引言
引言
1材料与方法1
1.1试验材料 1
1.1.1试验藻种1
1.1.2培养基1
1.1.3试剂2
1.2试验方法3
1．2．1培养方法3
1.2.1.1藻种的活化、扩培和传代3
1.2.1.2发酵培养基的优化3
1.2.1.3培养条件优化3
1.2.2常规分析测定方法5
1.2.2.1藻细胞pH的测定5
1.2.2.2藻细胞干重的测定5
1.2.2.3发酵液葡萄糖浓度测定5
1.2.2.4异养小球藻总蛋白测定方法5
1.2.2.5发酵液氮浓度测定6
2结果与分析7
2.1发酵培养基筛选7
2.2异养培养基缓冲体系优化8
2.3异养培养条件优化9
2.3.1单因素试验9
2.3.2正交试验12
2.4异养蛋白质积累实验13
2.4.1筛选影响蛋白核小球小藻异养蛋白质积累的因素13
2.4.2 5L发酵罐验证正交实验结果，促进蛋白质积累14
3讨论 16
3．1结论16
3．2展望16
致谢17
参考文献17
附录 实验设备18
蛋白核小球藻饵料发酵工艺研究
摘 要
微藻由于蕴含蛋白质、脂肪酸和色素等资源，近些年来被广泛应用于保健、医药、渔业等诸多行业。其中，蛋白核小球藻作为一种商业微藻，因富含优质蛋白质，被国际粮农组织列为理想的健康食品。在国外，藻蛋白的生产已商业化且产值上亿；我国小球藻的培养方式多为光能自养型，因其占地面积大、生长缓慢、后期不易采收等缺点难以满足藻蛋白市场。本实验希望通过对蛋白核小球藻的高密度、低成本培养来提高藻蛋白产量，满足市场需求。本试验对小球藻进行异养培养，从培养基成分和发酵条件两个方向出发，对培养过程中的 pH、接种量、装液量、种龄进行 *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
优化，来促进蛋白核小球藻的生长，提高藻蛋白产量。并进行放大试验。确定了Endo培养基和磷酸氢二钠磷酸二氢钠缓冲体系。通过单因素和正交试验优化确定了异养蛋白核小球藻的发酵条件：装液量150ml、接种量1.5g/L、种龄72h、初始pH6.5。并且筛选了影响蛋白核小球藻异养蛋白质积累的因素并进行正交试验优化，发现葡萄糖对蛋白质积累影响显著最后利用 5 L 发酵罐进行扩大培养，蛋白质含量较高。
STUDY ON THE FERMENTATION OF CHLORELLA PROTOTHECOIDES
ABSTRACT
Microalgae are widely used in health care, medicine,Many industries such as fisheries. Among them, protein nucleus chlorella as a commercial microalgae, because it is rich in highquality protein, is listed as an ideal healthy food by the International Food and Agriculture Organization. In foreign countries, the production of algal protein has been commercialized and its output value is over 100 million; the cultivation method of chlorella in China is mostly light energy autotrophic, which is difficult to meet the algal protein because of its large area, slow growth, and difficulty in late harvest. market. This experiment hopes to increase the production of algal protein through highdensity and lowcost cultivation of Chlorella nucleatum to meet market demand. In this experiment, chlorella was cultured heterotrophically. From the two directions of medium composition and fermentation conditions, the pH, inoculation amount, liquid volume and seed age during the cultivation process were optimized to promote the growth of Chlorella nucleatum. Growth and increase algae protein production. And conduct an enlargement test. The Endo medium and disodium hydrogen phosphatesodium dihydrogen phosphate buffer system were determined. The fermentation conditions of the heterotrophic protein Chlorella vulgaris were determined by single factor and orthogonal test optimization: the filling volume was 150ml, the inoculation volume was 1.5g / L, the seed age was 72h, and the initial pH was 6.5. The factors affecting the accumulation of heterotrophic proteins of Chlorella nucleus were screened and optimized by orthogonal test. It was found that glucose had a significant effect on protein accumulation. Finally, the 5 L fermentor was used to expand the culture and the protein content was higher.

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