目录
摘要Ⅱ
关键词 Ⅱ
AbstractⅢ
引言
引言1
1 文献综述2
1.1 热休克蛋白分类2
1.2 热休克蛋白生物学功能2
1.2.1 分子伴侣作用2
1.2.2 自稳作用2
1.2.3 免疫协同作用2
1.2.4 生长发育3
1.2.4 细胞凋亡3
1.3 热休克反应进化上的局限性3
1.4 分子伴侣辅因子3
2 材料与方法4
2.1 实验材料4
2.2 灵芝HSP20基因家族候选蛋白质的获取与鉴定4
2.2.1 直接获取蛋白序列4
2.2.2 间接获取蛋白序列4
2.2.3 候选蛋白的鉴定及保守结构预测4
2.3 HSP20蛋白序列比对和三级结构预测4
2.4 理化性质分析及信号肽鉴定5
2.5 系统进化树构建5
3 结果与分析5
3.1 灵芝HSP20基因家族的鉴定及保守结构预测5
3.2 蛋白序列比对和三级结构分析6
3.3 灵芝HSP20蛋白理化性质8
3.4 HSP20基因系统发育分析9
4 小结与讨论11
4.1 小结11
4.2 讨论12
致谢14
参考文献15
灵芝全基因组HSP20基因的鉴定与生物信息学分析
摘 要
因为热休克蛋白(HSP)种类和功能的多样性再加上其作用范围的广泛性，所以阐述热休克蛋白发展、分类和生物学作用是很有必要的。通过对热休克蛋白研究的概括，会更容易发现今后的热点研究方向且对本文的理解也有极大的促进作用。目前多数研究集中在HSP20、HSP70和HSP90蛋白上，其中对真菌HSP20蛋白的研究少之又少。因此本文选用具有 *51今日免费论文网|www.51jrft.com +Q: *351916072* 
极大药用价值的灵芝(G. Lucidum)作为对象，通过生物信息学的方法对灵芝全基因组进行了HSP20基因鉴定与分析。一共鉴定出8个具有HSP20蛋白保守结构域的灵芝HSP20蛋白。由于HSP20蛋白最初是单体形式，而当其发挥功能的时候常以多聚体的形式存在，所以本文对多聚体形式也进行了预测，共预测出2种不同的多聚体。通过本文的研究，有望能够揭开灵芝热休克反应(HSR)分子机制研究的序幕，并对今后小热激蛋白(sHSPs)的研究提供帮助。
GENOMEWIDE IDENTIFICATION AND BIOINFORMATICS ANALYSIS OF HSP20 GENE FAMILY IN GANODERMA LUCIDUM
ABSTRACT
Because of the diversity of types, functions and wide range of influence in heat shock proteins (HSP), it is necessary to illustrate the development, classification and biological functions of heat shock proteins. By summarizing the research of heat shock proteins, it will be easier to find the hot research direction in the future and it will greatly promote the understanding of this paper. At present, most of the researches focus on HSP20, HSP70 and HSP90 proteins. But researches on HSP20 proteins of fungi are few. Therefore, Ganoderma lucidum (G. Lucidum), which has great medicinal value, was selected as the object of this study. The HSP20 gene of G. Lucidum was identified and analyzed by the method of bioinformatics. Eight G. Lucidum HSP20 proteins with conserved HSP20 proteins domain were identified in this study. Because HSP20 proteins are in the form of monomer at first, and often exist in the form of polymer when they play theirs function. So this paper also forecasted the form of polymer in G. Lucidum. Then the author predicted two different kinds of polymer. Through this study, it is expected to open the prelude of the molecular mechanism of heat shock response (HSR)of G. Lucidum. Finally it will provide help for the research of small heat shock proteins (sHSPs)in the future.

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