目录
摘要 II
关键词 II
Abstract III
引言
引言 1
1 材料与方法 2
1．1 方案论证 2
1．2 实验材料 3
1．2．1 材料及试剂 3
1．2．2 实验仪器 3
1．3 实验方法 3
1．3．1 细胞存活率实验 3
1．3．2 细胞匀浆MDA、CAT及ROS检测 4
1．3．3 免疫印迹检测蛋白表达水平 4
1．3．3 统计学分析 4
2 结果与分析 5
2．1 实验结果 5
2．1．1 姜黄素对鸡肝细胞存活率的影响 5
2．1．2 姜黄素对鸡肝细胞氧化应激产物的影响 6
2．1．3 姜黄素对鸡肝细胞Caspase3、Bcl2、BAX蛋白表达水平的影响 7
2．2 结果分析 7
2．2．1 姜黄素对鸡肝细胞存活率的影响 7
2．1．2 姜黄素对鸡肝细胞氧化应激产物的影响 8
2．1．3 姜黄素对鸡肝细胞Caspase3、Bcl2、BAX蛋白表达水平的影响 9
3 讨论 9
参考文献 11
姜黄素对H2O2和LPS诱导的鸡肝脏细胞抗氧化能力的影响
摘 要
本课题研究重点实验项目旨在研究姜黄素对双氧水（H2O2）和脂多糖（LPS）)诱导鸡肝脏细胞氧化应激损伤的保护作用及因氧化应激所致细胞内膜损伤的功能抑制和细胞保护机理,探究可能的抑制作用和保护机制。实验取新鲜鸡的肝脏细胞，分别用H2O2和LPS诱导细胞氧化应激损伤模型，第一大组为空白对照组、H2O2诱导模型组、H2O2+5μmol/L姜黄素组、H2O2+10μmol/L姜黄素组、H2O2+20μmol/L姜黄素组，第二大组为空白对照组、LPS诱导模型组、LPS+5 μmol/L姜黄素组、LPS+10 μmol/L姜黄素组、LPS+20μmol/L姜黄素组，使用CCK8试剂盒检测姜黄素对细胞存活率的影响，相关试剂盒检测细胞匀浆中的氧化应激产物，免疫印迹法检测 *51今日免费论文网|www.jxszl.com +Q: ￥351916072￥ 
Caspase3、Bcl2、BAX以及Keap1蛋白表达水平。实验结果显示，H2O2和LPS均能诱导鸡肝脏细胞氧化应激损伤；随着不同剂量的姜黄素能够明显增加细胞活率，且剂量越高存活率越高；随着姜黄素的增加，每组细胞产生的MDA、ROS的含量明显减少，而CAT的含量下降。差异均有统计学意义；免疫印迹结果显示姜黄素可以拮抗H2O2和LPS诱导的细胞氧化应激所致的细胞凋亡，以达到降低鸡肝脏细胞内活性氧的水平的效果。实验说明姜黄素能增强鸡肝细胞的抗氧化能力。
Effects of curcumin on antioxidant capacity of H2O2 and LPS induced chicken liver cells
Abstract
The purpose of this experiment was to study the protective effect of curcumin on oxidative stress injury induced by hydrogen peroxide (H2O2) and LPS in chicken liver cells, and to explore its possible mechanism of action. Experiment for fresh chicken liver cells, cells induced by H2O2 and LPS respectively oxidative stress damage model, the first big group for blank control group, the H2O2 induction model group, H2O2 + 5 mu mol/L the curcumin group, H2O2 + 10 μmol/L the curcumin group, H2O2 + 20μ mol/L curcumin group, the secondlargest group as blank control group, LPS induced model group, the LPS + 5 μmol/L curcumin group, the LPS + 10 μmol/L curcumin group, the LPS + 20 μmol/L curcumin group, CCK8 was used to detect the effect of curcumin on the cell survival rate, the relevant kits were used to detect the oxidative stress products in the cell homogenate, and western blot was used to detectdd the protein expression levels of Caspase3, bcl2, BAX and Keap1. The experimental results showed that both H2O2 and LPS could induce oxidative stress injury of chicken liver cells. The comparison between the experimental group and the induced group showed that curcumin could significantly increase the cell survival rate, reduce the level of reactive oxygen species, reduce the content of malondialdehyde (MDA) and enhance the activity of catalase (CAT). The differences were statistically significant. Western blot results showed that curcumin could antagonized apoptosis induced by oxidative stress induced by H2O2 and LPS, so as to promote nuclear translocation of Nrf2 in hepatocytes, reduce the level of reactive oxygen species in cells, and thus reduce oxidative stress damage. To conclude, curcumin can reduce the level of reactive oxygen in chicken liver cell and enhence oxidation resistance of chicken liver cell.

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