目录
摘要Ⅲ
关键词Ⅲ
AbstractⅣ
引言
1选题背景5
2文献综述6
3方案论证9
4试验论述10
4.1试验材料10
4.1.1试验动物10
4.1.2主要试剂及仪器10
4.2试验方法10
4.2.1攻击行为的录制及观察10
4.2.2攻击行为的统计分析10
4.2.3攻击行为最强最弱猪的筛选10
4.2.4基因组DNA的提取11
4.2.5预测DRD2基因的核心启动子区11
4.2.6引物设计12
4.2.7 聚合酶链反应(PCR)条件及产物分析12
4.2.8测序结果分析13
4.2.9数据处理及分析13
5结果与分析14
5.1 攻击性强弱仔猪的行为上的差异14
5.2 猪DRD2基因序列分析14
5.3 猪DRD2基因启动子区生物信息学分析15
5.3.1 DRD2基因核心启动子区的预测15
5.3.2 Promoter 2.0预测结果15
5.3.3 猪DRD2基因启动子区 CpG 岛预测结果16
5.3.4攻击行为强和攻击行为弱的猪DRD2基因的差异SNP16
5.3.5 猪DRD2基因启动子区转录因子结合位点预测结果16
5.4 猪DRD2基因与攻击行为相关的SNP筛选及相关性分析17
6讨论19
6.1各栏中攻击性最强和最弱仔猪在攻击行为上的差异19
6.2猪DRD2基因启动子区生物信息学分析19
6.3 DRD2基因启动子区与猪攻击行为的关系19
致谢21
参考文献22猪DRD2基因启动子区SNP与断奶混群后攻击行为的相关性研究
摘要
为提高生猪养殖效益提高栏位利用率，养殖场多对断奶仔猪进行混群，但混群后猪群内的攻击行为降低了动物福利水平。多巴胺（Dopamine，DA）是哺乳动物大脑 *51今日免费论文网|www.jxszl.com +Q: ^351916072# 
中的神经递质，需要依靠膜受体来实现多种功能。前人研究表明多巴胺受体基因DRD2与哺乳动物攻击行为之间有关联，并且启动子区对基因的表达有着显著的影响。为探究猪DRD2基因启动子区单核苷酸多态性（SNP）与猪断奶混群后攻击行为强弱之间的相关性，本试验通过观察500头苏淮猪断奶仔猪在51栏中混群后36小时内的行为，对每头仔猪的攻击行为数据进行统计分析，选择出各栏中攻击性最强和攻击性最弱的仔猪共102头，采取耳组织样本，提取DNA。利用聚合酶链式反应（PCR）扩增技术，设计引物对DRD2基因启动子区进行扩增并测序，将所得数据进行与猪攻击行为强弱相关的SNP位点筛选。利用SPSS软件对SNP位点等位基因与攻击性强弱进行相关性分析。结果显示，猪DRD2基因启动子区SNP位点与断奶混群后攻击性强弱之间没有显著差异（P > 0.05）。
IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF PORCINE DRD2 PROMOTER REGION ASSOCIATED WITH AGGRESSIVE BEHAVIOR OF WEANED PIGS AFTER GROUP MIXING
ABSTRACT
In order to improve the efficiency of pig production, farmers often perform group mixing for weaned piglets, but aggressive behavior of piglets after mixing reduce the level of animal welfare. Dopamine (DA) is a neurotransmitter in mammalian brain, which needs membrane receptors to perform many functions. Previous studies have shown a strong link between dopamine receptor gene DRD2 and aggression in mammals， and that the promoter region has a significant effect on gene expression. To investigate the correlation between single nucleotide polymorphisms (SNP) of DRD2 promoter region and aggression of weaned pigs after mixing in this experiment, a total of 500 Suhuai weaned pigs were mixed in 51 pens after weaning for 36 hours, and the most aggressive pigs and the least aggressive pigs were selected in each pen. Ear samples of these pigs were taken to extract genomic DNA. The promoter region of DRD2 gene was amplified by the polymerase chain reaction (PCR) and sequenced. The correlation between the SNPs and the aggressiveness of pigs was examined by chisquare test in SPSS software. Using SPSS software to analyze the correlation between SNP allele and aggressiveness. The results showed that there was no significant difference between SNP of DRD2 gene promoter region and aggressiveness of pigs after mixing (P > 0.05).

原文链接：http://www.jxszl.com/yxlw/dwyx/605124.html