目录
目录
摘 要 I
ABSTRACT II
第一章 文献综述 1
前言 1
1 尿素缓释技术 1
2 缓释尿素的效用 3
3 存在的问题 3
4 缓释尿素的评价方法 4
5 缓释尿素的发展展望 4
6 本研究的目的及意义、技术路线 5
6.1 目的及意义 5
6.2 技术路线 5
第二章 试验研究 6
引言
1　材料与方法 6
1.1　试验材料 6
1.2 试验设计 7
1.3 试验方法 7
1.4 指标测定 9
1.5 数据分析 10
2 结果与分析 10
2.1 缓释尿素对瘤胃氨氮水平的影响 10
2.2 缓释尿素对瘤胃pH水平的影响 10
2.3 缓释尿素对瘤胃VFA水平的影响 11
3 讨论 12
3.1 不同种类缓释尿素对体外发酵氨氮浓度的影响 12
3.2 缓释尿素对反刍动物瘤胃内环境pH的影响 12
3.3 缓释尿素对反刍动物瘤胃VFA的影响 13
第三章 结论与展望 15
参考文献 16
致 谢 18
体外试验评价不同来源缓释尿素氨的释放速率
摘 要
目前市场上缓释尿素种类多，缓释效果不一。本试验以普通尿素为对照，通过体外发酵试验探究4种不同缓释尿素产品（B、C、D、E）氨的释放速率，进而确定不同缓释尿素产品的缓释效果。试验选取选取4只体况良好，安装有瘤胃瘘管的雄性育肥湖羊（平均体重25±1 kg），通过瘤胃瘘管采集瘤胃内容物，挤压并过滤出瘤胃液，将缓冲液与瘤胃液按9:1比例混合用于发酵试验。在不同时间采集发酵液，测定氨氮浓度。研究结果发现，体外发酵3 h时，缓释尿素B和D氨浓度显著低于普通尿素；9 h时，缓释尿素B和E氨浓度显著低于普通尿素。12 h时，缓释尿素B氨浓度显著低于普通尿素及其他3种缓释尿素，缓释尿素E氨浓度低于普通尿素；24  *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
h时，各处理组间氨浓度均无显著差异。综上所述，缓释尿素B与普通尿素和其他缓释尿素产品相比具有良好的缓释效果，具有在生产中大规模使用的潜力，能否完全替代普通尿素产生更高的经济效益需后续体内试验验证。
EVALUATION OF DIFFERENT SLOWRELEASE UREA PRODUCTS USING IN VITRO FERMENTATION METHODS
ABSTRACT
At present, there are many kinds of slow release urea in the market, and the slow release effect is different. In this experiment, the release rate of ammonia of four different sustainedrelease urea products (B, C, D, E) was investigated through in vitro fermentation test with ordinary urea as the control, and the sustainedrelease effect of different sustainedrelease urea products was determined. Four male fattening Hu sheep (average body weight 25±1kg) with rumen fistulas were selected, rumen contents were collected through rumen fistulas, rumen fluid was extracted and filtered out, and the buffer fluid and rumen fluid were mixed in a 9:1 ratio for fermentation test. The fermentation broth was collected at different time to determine the concentration of ammonia nitrogen. The results showed that the ammonia concentrations of B and D in slowrelease urea were significantly lower than those of normal urea after 3h of in vitro fermentation. At 9h, the ammonia concentrations of B and E in slowrelease urea were significantly lower than those in normal urea. At 12h, the ammonia concentration of slowrelease urea B was significantly lower than that of the other four kinds of slowrelease urea, and the ammonia concentration of slowrelease urea E was also lower than that of common urea. At 24h, there was no difference in ammonia concentration among all treatment groups. In conclusion, compared with ordinary urea and other sustainedrelease urea products, sustainedrelease urea B has a good sustainedrelease effect and has the potential to be used on a large scale in production. Whether it can completely replace ordinary urea and produce higher economic benefits needs to be verified by subsequent in vivo and feeding tests.

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